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SPTBN2 Antibody [SPTBN2/1583]

SPTBN2 Antibody [SPTBN2/1583]

Manufacturer	ProSci
Category	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Carcinoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Calcium Binding Protein Markers Interneuron Markers
Type	Antibody Monoclonal
Specific against	Human
Format	Liquid
Applications	WB, FC, IF, IHC-P
Amount	100 ug
Host	Mouse
Item no.	PRS-33-752-100ug
eClass 6.1	32160702
eClass 9.0	42030590
Available
Shipping	blue ice or RT
By Research Area	Neuroscience, Signal Transduction
Immunogen	Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
Applications	Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml IF: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 min at RT WB: 0.5-1 ug/ml The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	SPTBN2/1583
Isotype	IgG2b, kappa
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SPTBN2
Ncbi Gene Id #	6712
User Note	Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol	SPTBN2
Ncbi Official Full Name	spectrin beta, non-erythrocytic 2
Ncbi Organism	homo sapiens
Swissprot #	O15020
Background	Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alphaII (fodrin alpha) and spectrin betaI (fodrin beta) are present in other somatic cells. SPTBN2/Spectrin betaIII is highly homologous to both spectrin betaI and spectrin betaII. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.
1st Image Caption	IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1583). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2nd Image Caption	SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1583). Confirmation of Integrity and Purity of the Antibody.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 7/4/2024