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Comparison

Bcl-2 Antibody

Item no. PRS-3335-0.02mg
Manufacturer ProSci
Amount 0.02 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IF, ELISA, IHC-P
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
Citations Lockshin et al. Cell Death Differ. 2000; 7:2-7.
Cory et al. Oncogene 2003; 22:8590-607.
Heiser et al. Exp. Geron. 2004; 39:1125-35.
Hockenbery et al. Nature 1990; 348:334-6.
NCBI BCL2
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Bcl-2 Antibody: Bcl-2,PPP1R50,Apoptosis regulator Bcl-2
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Bcl-2antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 26kD
Observed: 26 kD
Manufacturer - Research Area
Apoptosis, Autophagy, Cancer
Background
Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
By Type
Apoptosis, Autophagy, Cancer
Badges
Trial Size, IAV
Modifications
None
Protein Gi #
20072668
Purification
Bcl-2 Antibody is affinity chromatography purified via peptide column.
Isoforms
Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD). Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD). Rat Bcl-2 has one isoform (236aa, 27kD). 3337 can detect all isoforms of human, mouse and rat
Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain.  Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. 

Positive Control 1
PRS-1288-0.1mg
Positive Control 2
PRS-1305-0.1mg
Positive Control 3
PRS-10-401-4um
Positive Control 4
PRS-1219-0.1mg
Positive Control 5
PRS-1208-0.1mg
Concentration
1 mg/mL
Buffer
Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note
WB: 1-2μ g/mL; IHC-P: 2 μ g/mL; IF: 10 μ g/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples ; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%), Chicken: (93%)
Immunogen
Anti-Bcl-2 antibody (3335) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bcl-2.

The immunogen is located within the first 50 amino acids of Bcl-2.
Clonality
Polyclonal
NCBI Official Name
B-cell CLL/lymphoma 2
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg
Available: In stock
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