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Bim Antibody

Bim Antibody

Item no.	PRS-3405-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Quantity options	0.02 mg 0.1 mg
Category	Oncology Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies By Organ Colorectal Cancer Testicular & Prostate Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	O'Connor et al. EMBO J. 1998; 17:384-395. Hsu et al. Mol. Endocrinol. 1998; 12:1432-40. Chen et al. CDK inhibitors upregulate BH3-only proteins to sensitize human myeloma cells to BH3 mimetic therapies. Cancer Res. 2012;72(16):4225-37. PMID: 22693249 Tsuchiya et al. Bcl-2 homology domain 3-only proteins Puma and Bim mediate the vulnerability of CA1 hippocampal neurons to proteasome inhibition in vivo. Eur J Neurosci. 2011;33(3):401-8.PMID: 21198986 Han et al. Regulation of mitochondrial apoptotic events by p53-mediated disruption of complexes between antiapoptotic Bcl-2 members and Bim. J Biol Chem. 2010;285(29):22473-83.PMID: 20404322 Chen et al. Bim upregulation by histone deacetylase inhibitors mediates interactions with the Bcl-2 antagonist ABT-737: evidence for distinct roles for Bcl-2, Bcl-xL, and Mcl-1. Mol Cell Biol. 2009;29(23):6149-69. PMID: 19805519 Ulett et al. Mechanisms of group B streptococcal-induced apoptosis of murine macrophages. J Immunol. 2005;175(4):2555-62.PMID: 16081829 Dai et al. Disruption of Src function potentiates Chk1-inhibitor-induced apoptosis in human multiple myeloma cells in vitro and in vivo. Blood. 2011;117(6):1947-57. PMID: 21148814 Yang et al. Promoter knock-in mutations reveal a role of Mcl-1 in thymocyte-positive selection and tissue or cell lineage-specific regulation of Mcl-1 expression. J Immunol. 2009 ;182(5):2959-68. PMID: 19234191 Han et al. Degradation of Mcl-1 by granzyme B: implications for Bim-mediated mitochondrial apoptotic events. J Biol Chem. 2004;279(21):22020-9. PMID: 15014070 Han et al. Disruption of Mcl-1.Bim complex in granzyme B-mediated mitochondrial apoptosis. J Biol Chem. 2005;280(16):16383-92.PMID: 15713684 Han et al. Interrelated roles for Mcl-1 and BIM in regulation of TRAIL mediated mitochondrial apoptosis. J Biol Chem.2006;281(15):10153-63.PMID: 16478725 Han et al. Functional linkage between NOXA and Bim in mitochondrial apoptotic events. J Biol Chem. 2007;282(22):16223-31.PMID: 17374615 Han et al. Deregulation of mitochondrial membrane potential by mitochondrial insertion of granzyme B and direct Hax-1 cleavage. J Biol Chem.2010;285(29:22461-72. PMID: 20388708 Fung et al. Induction of autophagy during extracellular matrix detachment promotes cell survival. Mol Biol Cell. 2008;19(3):797-806. PMID: 18094039 Chen et al. Targeting SQSTM1/p62 induces cargo loading failure and converts autophagy to apoptosis via NBK/Bik. Mol Cell Biol. 2014;34(18):3435-49.PMID: 25002530 Tanno et al. Bim-dependent apoptosis follows IGFBP-5 down-regulation in neuroblastoma cells. Biochem Biophys Res Commun. 2006;351(2):547-52. PMID: 17067554 Dai et al. Targeting autophagy in multiple myeloma. Leuk Res. 2017;59:97-104. PMID: 28599191 Dai Y. Targeting Autophagy in multiple myeloma. Virginia Commonwealth University VCU Scholars Compass. Dissertations. 2015.PMID:
NCBI	BCL2L11
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Bim Antibody: BAM,BIM,BOD,Bcl-2-like protein 11,Bcl2-interacting mediator of cell death,Bcl2-L-11
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Bim antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	Predicted: 22kD Observed: 22kD
Manufacturer - Research Area	Apoptosis, Cancer
Background	Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
By Type	Apoptosis, Cancer
Badges	Trial Size, IAV, Most Cited, Most Images
Modifications	None
Protein Gi #	18202042
Purification	Bim Antibody is affinity chromatography purified via peptide column.
Isoforms	Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). 3405 only detect isoform EL.
Validation	Independent Antibody Validation in Cell lines (Figure 2) shows similar BIM expression profile in human and mouse cell lines detected by two independent anti-BIM antibodies that recognize different epitopes, 2065 against N-terminus domain and 3405 against central domain. BIM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. KD validation (Figure 7, 9, 10): Anti-BIM antibody (3405) specificity was further verified by BIM specific knockdown. BIM signal in 293, U937, Jurkat and U266 cells transfected with BIM siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs or shRNA. Regulated expression validation (Figure 8): BIM expression detected by anit-BIM antibodies (3405) was up-regulated by flavopiridol treatment, which was blocked by Cycloheximide in U266 cells.
Positive Control 1	PRS-1204-0.1mg
Positive Control 2	PRS-17-004-1pack
Positive Control 3	PRS-1201-0.1mg
Positive Control 4	PRS-1224-0.1mg
Concentration	1 mg/mL
Buffer	Bim Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note	WB: 5 μ g/mL; ICC: 10 μ g/mL; IF: 20 μ g/mL. Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Homology	Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen	Anti-BIM antibody (3405) was raised against a peptide corresponding to amino acids near the center of human BIM. The immunogen is located within amino acids 80-130 of BIM.
Clonality	Polyclonal
NCBI Official Name	BCL2-like 11 (apoptosis facilitator)
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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