Caspase-8 Antibody

Blue Ice

55 kDa

Caspase-8 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.

Trial Size

15718704

PRS-1205-0.1mg

PRS-1406-0.1mg

PRS-10-901-4um

1 mg/mL

Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ g/mL and immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.
The immunogen is located within the last 50 amino acids of Caspase-8.

caspase 8, apoptosis-related cysteine peptidase

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.