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Apaf-1 Antibody [13F11]

Apaf-1 Antibody [13F11]

Item no.	PRS-36-176-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Antibodies Cell Biology Cell Status Apoptosis Immunoprecipitation Western Blot ELISA Immunocytochemistry Cardiovascular Research Ischemia & Reperfusion Injury Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IP, ICC, ELISA
Clone	13F11
Specific against	Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rat
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
NCBI	Apaf1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Apoptosis Protease-activating Factor 1
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Stable for at least 1 year after receipt when stored at -20˚ C.
Manufacturer - Research Area	Apoptosis, Cancer
Background	Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
By Type	Apoptosis, Cancer
Modifications	None
Protein Gi #	Q9EPV5
Purification	>95% (SDS-PAGE)
Concentration	1 mg/ml
Buffer	Liquid. In PBS containing 0.02% sodium azide.
Application Note	Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
Manufacturer - Specificity	Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
Immunogen	Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
Clonality	Monoclonal
NCBI Official Name	apoptotic peptidase activating factor 1
NCBI Organism	Rattus norvegicus
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 1/1/2026

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