Comparison

Beta-actin Antibody

Item no. PRS-3777-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IF, ICC, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
Citations Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
NCBI ACTB
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Beta-actin Antibody: BRWS1,PS1TP5BP1,Actin,cytoplasmic 1,Beta-actin,b-actin
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Beta-actin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 41 kDa
Observed: 41 kDa
Manufacturer - Research Area
Homeostasis
Background
Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
By Type
Homeostasis
Badges
Trial Size
Modifications
None
Protein Gi #
12803203
Purification
Beta-actin Antibody is affinity chromatography purified via peptide column.
Positive Control 1
Positive Control 2
Concentration
1 mg/mL
Buffer
Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note
Beta-actin antibody can be used for the detection of Beta-actin by Western blot at 0.5 - 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Homology
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Rabbit: (100%), Pig: (100%), Horse: (100%), Chicken: (100%), Guinea pig: (100%), Bovine: (100%)
Immunogen
Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beta-actin.

The immunogen is located within the last 50 amino acids of Beta-actin.
Clonality
Polyclonal
NCBI Official Name
actin, beta
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
available

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