Comparison

GAPDH Antibody

Item no. PRS-3783-0.02mg
Manufacturer ProSci
Amount 0.02 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, ELISA, IHC-P
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
Citations Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
NCBI GAPDH
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias GAPDH Antibody: G3PD,GAPD,HEL-S-162eP,CDABP0047,OK/SW-cl.12,Glyceraldehyde-3-phosphate dehydrogenase,Peptidyl-cysteine S-nitrosylase GAPDH,GAPDH
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
GAPDH antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 36 kD

Observed: 36 kD
Manufacturer - Research Area
Signal Transduction, Neuroscience, Homeostasis
Background
GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
By Type
Signal Transduction, Neuroscience, Homeostasis
Badges
Trial Size
Modifications
None
Protein Gi #
120649
Purification
GAPDH Antibody is affinity chromatography purified via peptide column.
Positive Control 1
Positive Control 2
Positive Control 3
Concentration
1 mg/mL
Buffer
GAPDH Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note
WB: 0.5-2 μ g/mL; IHC-P: 1-2 μ g/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Homology
Predicted species reactivity based on immunogen sequence: Sheep: (87%), Rabbit: (87%), Pig: (87%), Bovine: (87%), Chicken: (87%)
Immunogen
GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human GAPDH.

The immunogen is located within amino acids 60 - 110 of GAPDH.
Clonality
Polyclonal
NCBI Official Name
glyceraldehyde-3-phosphate dehydrogenase
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.02 mg
Available: In stock
available

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