PD-1 Antibody

blue ice or RT

Anti-PD-1 antibody (4065) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human PD-1.

The immunogen is located within amino acids 210-260 of PD-1.

Cat. No. 1204 - K562 Cell Lysate

Cat. No. 1208 - THP-1 Cell Lysate

Human PD-1 has 1 isoform (288aa, 32kD). Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD). 4065 can detect human, mouse and rat.

Human PD-1 has 1 isoform (288aa, 32kD). Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD). 4065 can detect human, mouse and rat.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4065P – PD-1 Peptide

None

Q15116

5133

PDCD1

Homo sapiens

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

Ishida et al. EMBOJ. 1992; 11:3887-95.

Nishimura et al. Immunity 1999; 11:141-51

Figure 2 KD Validation in HeLa Cells
Loading: 10 μ g of HeLa WT cell lysates or PD-1 KD cell lysates. Antibodies: PD-1, 4065 (4 μ g/mL) and beta-actin 3779 (1 μ g/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in THP-1 Cell Lysate in the (A) absence and (B) presence of blocking peptide
Loading: 15 μ g of lysates per lane.Antibodies: PD-1, 4065 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (4065) at 5 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 8 Immunohistochemistry Validation of PD-1 in Human Brain Tissue
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PD-1 antibody (4065) at 2.5 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.