IL-33 Antibody

Primary Antibodies

IL-33 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α /beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κ B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.

Trial Size

15559209

PRS-1369-0.1mg

1 mg/mL

IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 μ g/mL. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human IL-33.

The immunogen is located within amino acids 50 - 100 of IL-33.

interleukin 33

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.