Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Rat |
Format |
Liquid |
Applications |
WB, ELISA |
Amount |
0.1 mg |
Host |
Goat |
Item no. |
PRS-43-544-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Homology |
Expected Species Reactivity based on sequence homology: Mouse |
Immunogen |
The immunogen for this antibody is: C-QKEQHSDLDKSSIH |
Applications |
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Rat Colon lysates (calculated MW of 54.0kDa according to NP_036765.2). Recommended concentration: 0.1-0.3ug/ml. An additional faint band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. |
Specificity |
This antibody is expected to recognize all reported isoforms (NP_038667.2; NP_001157060.1; NP_001157061.1). |
Predicted Molecular Weight |
Approx 55kDa |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 43-544P - Peripherin 1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
Peripherin 1 |
Accession # |
NP_038667.2, NP_001157060.1, NP_001157061.1 |
Protein Gi # |
254675335 |
Ncbi Gene Id # |
19132 |
Ncbi Official Symbol |
Prph |
Ncbi Official Full Name |
peripherin |
Ncbi Organism |
Mus musculus |
Swissprot # |
P15331 |
Background References 1 |
McLean JR, Robertson J.Isoform-specific expression and ratio changes accompany oxidant-induced peripherin aggregation in a neuroblastoma cell line. |
1st Image Caption |
43-544 (0.1ug/ml) staining of Rat Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.