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TRIM28 Antibody

TRIM28 Antibody

Manufacturer	ProSci
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies CHiP
Type	Antibody Primary
Specific against	Human
Format	Liquid
Applications	FC, IF, IHC, ELISA
Amount	0.1 mg
Host	Goat
Item no.	PRS-45-162-0.1mg
eClass 6.1	32160702
eClass 9.0	32160702
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction , Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse
Immunogen	The immunogen for this antibody is: C-SSQELSGGPGDGP
Applications	Peptide ELISA: antibody detection limit dilution 1:2000.Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificity	.
Positive Control 1	Cat. No. 1211 - HepG2 Cell Lysate
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-162P - TRIM28 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TRIM28
Accession #	NP_005753.1
Protein Gi #	5032179
Ncbi Gene Id #	10155
Ncbi Official Symbol	TRIM28
Ncbi Official Full Name	tripartite motif containing 28
Ncbi Organism	Homo sapiens
Swissprot #	Q13263
Background References 1	Friedman JR, Fredericks WJ, Jensen DE, Speicher DW, Huang XP, Neilson EG, Rauscher FJ 3rd. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996 Aug 15; 10(16):2067-78.
1st Image Caption	45-162 (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
2nd Image Caption	45-162 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
3rd Image Caption	45-162 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
4th Image Caption	45-162 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 7/4/2024