Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Rat |
Format |
Liquid |
Applications |
WB, ELISA |
Amount |
0.1 mg |
Host |
Goat |
Item no. |
PRS-45-274-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Dog |
Immunogen |
The immunogen for this antibody is: C-ERNKKKQLPYYYLSPD |
Applications |
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85kDa band observed in Human Bone Marrow and Rat Stomach lysates (calculated MW of 77.7.0kDa according to rat NP_036954). Recommended concentration: 0.3-1ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1475 - Rat Stomach Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-274P - Arachidonate 5-lipoxygenase Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ALOX5 |
Accession # |
NP_000689.1 |
Protein Gi # |
4502057 |
Ncbi Gene Id # |
240 |
Ncbi Official Symbol |
ALOX5 |
Ncbi Official Full Name |
arachidonate 5-lipoxygenase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P09917 |
Background References 1 |
Kalayci O, Birben E, Sackesen C, Keskin O, Tahan F, Wechsler ME, Civelek E, Soyer OU, Adalioglu G, Tuncer A, Israel E, Lilly C. ALOX5 promoter genotype, asthma severity and LTC production by eosinophils. Allergy. 2006 Jan; 61(1):97-103. |
1st Image Caption |
45-274 (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.