Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Rat |
Format |
Liquid |
Applications |
WB, IHC, ELISA |
Amount |
0.1 mg |
Host |
Goat |
Item no. |
PRS-45-982-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Dog |
Immunogen |
The immunogen for this antibody is: C-RMKKLNSSDDGTQ |
Applications |
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3ug/ml. |
Specificity |
. |
Positive Control 1 |
Cat. No. 1202 - A431 Cell Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-982P - NRAS Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NRAS |
Accession # |
NP_002515.1 |
Protein Gi # |
4505451 |
Ncbi Gene Id # |
4893 |
Ncbi Official Symbol |
NRAS |
Ncbi Official Full Name |
neuroblastoma RAS viral (v-ras) oncogene homolog |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P01111 |
Background References 1 |
Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K. Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma. Cancer Epidemiol Biomarkers Prev. 2007 May; 16(5):991-7. |
1st Image Caption |
45-982 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
2nd Image Caption |
45-982 (0.01ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence. |
3rd Image Caption |
45-982 (0.1ug/ml) staining of Rat Intestines lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.