Ambra1 Antibody

Blue Ice

Predicted: 143kD

Observed: 143 kD

Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

Trial Size

166215833

PRS-1282-0.1mg

1 mg/mL

WB: 1-2 μ g/mL; IHC: 1-5 μ g/mL; IF: 20 μ g/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1.

The immunogen is located within the last 50 amino acids of Ambra1.

autophagy/beclin-1 regulator 1

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.