Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Mouse, Rat, Chicken |
Format |
Liquid |
Applications |
WB, IHC |
Amount |
0.1 mL |
Host |
Mouse |
Item no. |
PRS-50-257-0.1mL |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Immunogen |
Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. |
Specificity |
Specific for the ~145k Neurofilament M protein. |
Predicted Molecular Weight |
145 |
Purification |
Total IgG fraction |
Clonality |
Monoclonal |
Clone |
3H11 |
Isotype |
IgG1 |
Conjugate |
Unconjugated |
Storage Conditions |
Neurofilament NF-M antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
Nefm |
Accession # |
P12839 |
Protein Gi # |
128150 |
Ncbi Gene Id # |
24588 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
Nefm |
Ncbi Official Full Name |
neurofilament, medium polypeptide |
Ncbi Organism |
Rattus norvegicus |
Swissprot # |
P12839 |
Background |
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. |
Background References 1 |
Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. |
Background References 2 |
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. |
1st Image Caption |
Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.