Comparison

SCF Antibody

Item no. PRS-5165-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IF, ELISA, IHC-P
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
Citations Zhang et al. Up-regulation of the Ang II/AT1 receptor may compensate for the loss of gastric antrum ICC via the PI3k/Akt signaling pathway in STZ-induced diabetic mice. Mol Cell Endocrinol. 2016;423:77-86.PMID: 26773730
Wu et al. Diabetes-induced loss of gastric ICC accompanied by up-regulation of natriuretic peptide signaling pathways in STZ-induced diabetic mice. Peptides. 2013;40:104-11. PMID: 23352981
Reber et al. Euro. J. Pharmacology 2006; 533:327-40.
Jensen et al. Inflamm. Allergy Drug Targets 2007; 6:57-62.
Lukacs et al. J. Immunol. 1996; 156:3945-51.
NCBI KITLG
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias SCF Antibody: SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor
Available
Manufacturer - Type
Primary Antibodies
Manufacturer - Applications
WB: 1-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Country of Origin
USA
Shipping Temperature
Blue ice
Storage Conditions
SCF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 31kD
Observed: 36 kD (31kD + 4 N-linked Glycosylations)
Manufacturer - Research Area
Stem Cell
Background
SCF Antibody: Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
Badges
Trial Size
Protein Gi #
134289
Purification
SCF Antibody is affinity chromatography purified via peptide column.
Isoforms
Human SCF has 3 isoforms, including isoform 1 (273aa, 30.9kD), isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD). Mouse SCF has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD). Rat SCF also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD). 5165 can detect human and possibly detect mouse and rat.
Positive Control 2
Concentration
1 mg/ml
Buffer
SCF Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note
WB: 1-2 μ g/mL; IHC: 2.5 μ g/mL; IF: 20 μ g/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (71%)
Immunogen
Anti-SCF antibody (5165) was raised against a peptide corresponding to 18 amino acids near the center of human SCF.
The immunogen is located within amino acids 100-150 of SCF.
Clonality
Polyclonal
NCBI Official Name
KIT ligand
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Validation

Recombinant Protein Test (Figure 2): Anti-SCF antibodies (5165) detected human SCF recombinant protein at different concentrations.

Regulated expression validation (Figure 7 & 8): SCF expression detected by anti-SCF antibodies (5165) was up-regulated by angiotensin II (Ang II) treatment and was down-regulated by two inhibitors (Figure 7) or CNP treatment (Figure 8).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close