NANOG Antibody

Blue Ice

Predicted: 35 kD Observed: 35 kD

NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Trial Size

119609057

Human NANOG has 2 isoforms, isoform 1 (305aa, 34.6kD) and isoform 2 (289aa, 32.8kDa). Mouse NANOG has 2 isoforms, isoform 1 (305aa, 34.2kD) and isoform 2 (280aa, 31.9kD). Rat NANOG has 1 isoform (311aa, 35.1kD). 5585 can detect NANOG in human, mouse, and rat.

PRS-1476-0.1mg

NANOG Antibody is supplied in PBS containing 0.02% sodium azide.

Predicted species reactivity based on immunogen sequence: Bovine: (89%), Chicken: (94%)

Polyclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher.