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ENOA Antibody

ENOA Antibody

Manufacturer	ProSci
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human, Mouse
Format	Liquid
Applications	WB, FC, IF, IHC-P
Amount	400 ul
Host	Rabbit
Item no.	PRS-61-572-400ul
eClass 6.1	32160702
eClass 9.0	32160702
Available
Shipping	blue ice or RT
By Research Area	Cancer, Obesity, Signal Transduction
Homology	Predicted species reactivity based on immunogen sequence: Monkey
Immunogen	This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA.
Applications	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight	47 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ENO1
Accession #	P06733
Protein Gi #	119339
Ncbi Gene Id #	2023
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ENO1
Ncbi Official Full Name	Alpha-enolase
Ncbi Organism	Homo sapiens
Swissprot #	P06733
Background	ENO1 is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Background References 1	Cappello, P., Int. J. Cancer 125 (3), 639-648 (2009)
Background References 2	Wygrecka, M., Blood 113 (22), 5588-5598 (2009)
1st Image Caption	Western blot analysis in Jurkat, MCF-7, U251 cell line and mouse brain tissue lysates (35ug/lane).
2nd Image Caption	Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
3rd Image Caption	Confocal immunofluorescent analysis of ENOA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
4th Image Caption	ENOA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 6/6/2024