Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human |
Format |
Liquid |
Applications |
WB, IF, IHC-P |
Amount |
400 ul |
Host |
Rabbit |
Item no. |
PRS-63-122-400ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
This PDGFRB antibody is generated from rabbits immunized with human recombinant PDGFRB protein. |
Applications |
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
124 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PDGFRB |
Accession # |
P09619 |
Protein Gi # |
129890 |
Ncbi Gene Id # |
5159 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PDGFRB |
Ncbi Official Full Name |
Platelet-derived growth factor receptor beta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P09619 |
Background |
PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. |
Background References 1 |
Wu, H., et al., J. Biol. Chem. 278(42):40425-40428 (2003). |
Background References 2 |
Whiteman, E.L., et al., Endocrinology 144(9):3811-3820 (2003). |
Background References 3 |
Uren, A., et al., Oncogene 22(15):2334-2342 (2003). |
Background References 4 |
Steer, E.J., et al., Acta Haematol. 107(2):113-122 (2002). |
1st Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
2nd Image Caption |
Western blot analysis of anti-PDGFRB Pab in Y79 cell line lysates (35ug/lane). |
3rd Image Caption |
Confocal immunofluorescent analysis of PDGFRB Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.