Comparison

NETO1 Antibody

Item no. PRS-6469-0.02mg
Manufacturer ProSci
Amount 0.02 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IF, ELISA, IHC-P
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
Citations Michishita M, Ikeda T, Nakashiba T, et al. A novel gene, Btcl1, encoding CUB and LDLa domains is expressed in restricted areas of mouse brain. Biochem. Biophys. Res. Commun. 2003; 306:680-6.
Stöhr H, Berger C, Fröhlich S, et al. A novel gene encoding a putative transmembrane protein with two extracellular CUB domains and a low-density lipoprotein class A module: isolation of alternatively spliced isoforms in retina and brain. Gene 2002; 286:223-31
Straub C, Hunt DL, Yamasaki M, et al. Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1. Nat. Neurosci. 2011; 14:866-73.
Ng D, Pitcher GM, Szilard RK, et al. Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning. PLoS Biol. 2009; 7:e41
NCBI NETO1
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias NETO1 Antibody: BCTL1,BTCL1,Neuropilin and tolloid-like protein 1,Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
NETO1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 60 kDa
Observed: 60 kDa
Manufacturer - Research Area
Neuroscience
Background
NETO1 Antibody: Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.
By Type
Neuroscience
Badges
Trial Size
Modifications
None
Protein Gi #
20452470
Purification
NETO1 Antibody is affinity chromatography purified via peptide column.
Positive Control 1
Concentration
1 mg/mL
Buffer
NETO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note
NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Manufacturer - Specificity
NETO1 antibody is predicted to not cross-react with other NETO protein family members.
Immunogen
NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1.

The immunogen is located within amino acids 350 - 400 of NETO1.
Clonality
Polyclonal
NCBI Official Name
neuropilin (NRP) and tolloid (TLL)-like 1
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.02 mg
Available: In stock
available

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