Comparison

SPINK2 Antibody

Item no. PRS-6667-0.02mg
Manufacturer ProSci
Amount 0.02 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IF, ELISA, IHC-P
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
Citations Lee B, Park I, Jin S, et al. Impaired spermatogenesis and fertility in mice carrying a mutation in the Spink2 gene expressed predominantly in testes. J. Biol. Chem. 2011; 286:29108-17
Chen T, Lee TR, Liang WG, et al. Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor. Proteins 2009; 77:209-19.
Liddle RA. Pathophysiology of SPINK mutations in pancreatic development and disease. Endocrinol. Metab. Clin. North Am. 2006; 35:345-56.
NCBI SPINK2
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias SPINK2 Antibody: HUSI-II,Serine protease inhibitor Kazal-type 2,Acrosin-trypsin inhibitor
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
SPINK2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 15 kDa
Observed: 18 kDa
Manufacturer - Research Area
Stem Cell
Background
SPINK2 Antibody: Human serine proteinase inhibitor Kazal-type 2 (SPINK2) is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells. It contains a typical kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. SPINK2 plays a role in the pathogenesis of hereditary and chronic pancreatitis.
By Type
Stem Cell
Badges
Trial Size
Modifications
None
Protein Gi #
576796017
Purification
SPINK2 Antibody is affinity chromatography purified via peptide column.
Positive Control 1
Concentration
1 mg/mL
Buffer
SPINK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note
SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Homology
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Manufacturer - Specificity
SPINK2 antibody is predicted to not cross-react with other SPINK family members.
Immunogen
SPINK2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of mouse SPINK2.

The immunogen is located within the last 50 amino acids of SPINK2.
Clonality
Polyclonal
NCBI Official Name
serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
NCBI Organism
Mus musculus
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.02 mg
Available: In stock
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