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SMURF2 Antibody

SMURF2 Antibody

Item no.	PRS-7773-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Quantity options	0.02 mg 0.1 mg
Category	Antibodies Cell Biology Cell Status Immunohistochemistry Western Blot ELISA Immunofluorescence Senescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7. Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503. Lin X, Liang M, and Feng XH. Smurf2 is a ubiquitin E3 ligase mediating proteasomedependent degradation of Smad2 in transforming growth factor-beta signaling. J. Biol. Chem. 2000; 275:36818-22. Kavsak P, Rasmussen RK, Causing CG, et al. Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation. Mol. Cell 2000; 6:1365-75.
NCBI	SMURF2
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2,SMAD ubiquitination regulatory factor 2,hSMURF2
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	SMURF2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Molecular Weight	Predicted: 82 kDa Observed: 85 kDa
Manufacturer - Research Area	Signal Transduction
Background	SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1, 2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).
By Type	Signal Transduction
Badges	Trial Size
Modifications	None
Protein Gi #	12232397
Purification	SMURF2 antibody is affinity chromatography purified via peptide column.
Positive Control 1	PRS-1285-0.1mg
Concentration	1 mg/mL
Buffer	SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note	SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry at 2 μ g/mL. For Immunoflorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Manufacturer - Specificity	SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
Immunogen	SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2. The immunogen is located within amino acids 140 - 190 of SMURF2.
Clonality	Polyclonal
NCBI Official Name	SMAD specific E3 ubiquitin protein ligase 2
NCBI Organism	homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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