Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Mouse, Rat |
Format |
Liquid |
Applications |
WB, IF |
Amount |
0.1 mg |
Host |
Rabbit |
Item no. |
PRS-79-628-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer |
Immunogen |
LC3A antibody was raised against a peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A. |
Applications |
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
Specificity |
This antibody detects endogenous level of total LC3A protein. |
Predicted Molecular Weight |
18, 16 kDa |
Purification |
Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration |
1 mg/mL |
Storage Conditions |
Store antibody at -20˚ C for up to one year. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MAP1LC3A |
Accession # |
NP_115903.1 |
Protein Gi # |
14210522 |
Ncbi Gene Id # |
84557 |
Ncbi Official Symbol |
MAP1LC3A |
Ncbi Official Full Name |
microtubule-associated protein 1 light chain 3 alpha |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9H492 |
Background |
Probably involved in formation of autophagosomal vacuoles (autophagosomes). |
Background References 1 |
Hammarback JA, et al. Neuron 7 (1): 129-39, 1991. He H, et al. J. Biol Chem 278 (31): 29278-87, 2003. |
1st Image Caption |
Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody. |
2nd Image Caption |
Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody #21402. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.