Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
other |
Format |
Liquid |
Applications |
IHC, ELISA |
Amount |
0.02 mg |
Host |
Rabbit |
Item no. |
PRS-9275-0.02mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Infectious Disease, COVID-19 |
Immunogen |
Anti-SARS-CoV-2 (COVID-19) ORF3a antibody (9275) was raised against a peptide corresponding to 15 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3a protein.
The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3a protein. |
Applications |
IHC: 0.2 μ g/mL
Antibody validated: Immunohistochemistry in human samples. Antibody validated: SARS-CoV-2 (COVID-19) ORF3a antibody can detect 2 ng of free peptide at 1 μ g/mL in ELISA. All other applications and species not yet tested. |
Purification |
SARS-CoV-2 (COVID-19) ORF3a Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
SARS-CoV-2 (COVID-19) ORF3a antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
SARS-CoV-2 (COVID-19) ORF3a antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 9275P - SARS-CoV-2 (COVID-19) ORF3a Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Ncbi Official Symbol |
ORF3a |
Accession # |
P0DTC3 |
Protein Gi # |
1835921851 |
Ncbi Gene Id # |
43740569 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ORF3a |
Ncbi Official Full Name |
ORF3a protein |
Ncbi Organism |
Severe acute respiratory syndrome coronavirus 2 |
Swissprot # |
P0DTC3 |
Background |
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF3a forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. It up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. It induces apoptosis in cell culture and downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination (3). |
Background References 1 |
Gorbalenya. bioRxiv: 2020. |
Background References 2 |
Hui et al. Int J Infect Dis. 2020; 91:264-266. |
Background References 3 |
Lu et al. PNAS USA. 2006; 103:12540-12545. |
1st Image Caption |
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3a in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF3a antibody (9275, 0.2 μ g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung. |
2nd Image Caption |
Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) ORF3a Antibody, 9275. A direct ELISA was performed using SARS-CoV-2 ORF3a immunogen peptide (9275P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.