Comparison

Anti-WISP1 antibody (C-Term) (STJ71370) European Partner

Item no. STJ71370-100
Manufacturer St John's Laboratory
Amount 100 ug
Category
Type Antibody Primary
Applications WB, FC, IF, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Caprine
Isotype IgG
Conjugate/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Manufacturer - Type
Primary antibodies
Manufacturer - Applications
Pep-ELISA/WB/IF/FC
Manufacturer - Targets
CCN4
Storage Conditions
Store at-20°C on receipt and minimise freeze-thaw cycles.
Formulation
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA NA
Concentration
0.5 mg/mL
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Gene symbol
CCN4
Dilution range
Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 32-35kDa band observed in lysates of cell line CaCo-2 and approx. 40kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated MW of 30.7kDa according to NP_543028.1 and 40.3kDa according to NP_003873.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IF: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Immunogen Region
C-Term
Manufacturer - Specificity
This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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