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Anti-PU.1 antibody (Internal) (STJ71510)

Anti-PU.1 antibody (Internal) (STJ71510)

Item no.	STJ71510-100
Manufacturer	St John's Laboratory
Amount	100 ug
Category	Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC, ELISA
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus)
Host	Caprine
Isotype	IgG
Conjugate/Tag	Unconjugated
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Manufacturer - Type	Primary antibodies
Manufacturer - Applications	Pep-ELISA/WB/IHC
Manufacturer - Targets	SPI1
Storage Conditions	Store at-20°C on receipt and minimise freeze-thaw cycles.
Formulation	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA NA
Concentration	0.5 mg/mL
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Gene symbol	SPI1
Dilution range	Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30; 275 (26) :19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75µg/ml.
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Immunogen Region	Internal
Manufacturer - Specificity	This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 10/2/2025

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