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Comparison

Anti-S100A9 antibody (Internal) (STJ71826) European Partner

Item no. STJ71826-100
Manufacturer St John's Laboratory
Amount 100 ug
Category
Type Antibody Primary
Applications WB, FC, IF, IHC, ELISA
Specific against Human (Homo sapiens)
Host Caprine
Isotype IgG
Conjugate/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Manufacturer - Type
Primary antibodies
Manufacturer - Applications
Pep-ELISA/WB/IHC/IF/FC
Manufacturer - Targets
S100A9
Storage Conditions
Store at-20°C on receipt and minimise freeze-thaw cycles.
Formulation
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA NA
Concentration
0.5 mg/mL
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Gene symbol
S100A9
Dilution range
Peptide ELISA: antibody detection limit dilution 1:8000.
WB: Approx 13-14kDa band observed in Human Bone Marrow lysates and approx 14-15kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2kDa according to NP_002956.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Lung. Recommended concentration: 2-4µg/ml.Paraffin embedded Human Spleen. Recommended concentration: 6-7µg/ml.
IF: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7, U2OS and THP-1 cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Immunogen Region
Internal

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug
Available: In stock
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