Anti-TRIM29/ATDC antibody (Internal) (STJ72127)

Pep-ELISA/WB/IF/FC/IHC

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room tempetature.
IHC: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75µg/ml.
IF: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Internal