Comparison

Anti-PRDM14 antibody (414-427) (STJ72514) European Partner

Item no. STJ72514-100
Manufacturer St John's Laboratory
Amount 100 ug
Category
Type Antibody Primary
Applications FC, IF, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Dog (Canine, Canis lupus familiaris), Pig (Porcine, Sus scrofa domesticus), Cow (Bos taurus)
Host Caprine
Isotype IgG
Conjugate/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Manufacturer - Type
Primary antibodies
Manufacturer - Applications
Pep-ELISA/IF/FC
Manufacturer - Targets
PRDM14
Storage Conditions
Store at-20°C on receipt and minimise freeze-thaw cycles.
Formulation
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA NA
Concentration
0.5 mg/mL
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Gene symbol
PRDM14
Dilution range
Peptide ELISA: antibody detection limit dilution 1:16000.
WB: Preliminary experiments gave an approx 40kDa band in Human Heart lysates after 0.1-1µg/ml antibody staining and this band was successfully blocked by incubation with the immunizing peptide. Preliminary experiments also gave an approxand an approx 48-50kDa band in Human Lung and Kidney lysates and in nuclear lysates of cell liines Jurkat, HEK293 and MCF7. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 64.1kDa according to Human NP_078780.1.
IF: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Immunogen Region
414-427

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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