« Back
Home /
Anti-Mouse CD252 (OX40L) - Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD252 (OX40L) - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-C2336-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IHC, other, IV
Clone	RM134L
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
NCBI	22164
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. Each lot of this antibody is quality control tested.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Immune Checkpoints\|Purified in vivo Functional Grade\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD252
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.
Background	CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.
Function	B-cell/T-cell interaction, costimulation
Other Applications Reported In Literature	Additional reported applications (for the relevant formats) include: Immunohistochemical Staining Western Blotting
PubMed	CD252
Ligand/Receptor	OX40(CD134)
Manufacturer - Research Area	Costimulatory Molecules, Immunology
Manufacturer - Specificity	Clone RM134L recognizes an epitope on mouse CD252.
RRID	AB_2737465
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD252 is expressed on activated B cells and antigen presenting cells.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Rat NRK-52E cell line transfected with mouse OX-40L gene
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

LEIN-C2336-1.0mg-safety-datasheet.pdf