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Anti-Human/Mouse Integrin β7 - Purified in vivo GOLD™ Functional Grade

Anti-Human/Mouse Integrin β7 - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-I-1141-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IP, other, IV
Clone	FIB504
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905 2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26 3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8 4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93 5. Hadley GA Higgins JM. (2014) Adv Exp Med Biol. 819:97–110 6. Farstad IN., et al. (1996) Immunology. 89:227–37 7. Jaensson E., et al. (2008) J Exp Med. 205:2139–49 8. Allez M, et al. (2002) Gastroenterology. 123(5):1516-26 9. Schön MP, et al. (1999) J Immunol. 162(11):6641-9 10. Stefanich EG, et al. (2011) Br J Pharmacol. 162(8):1855-1870 11. Smids C, et al. (2017) J Crohns Colitis. 11(4):500-508
NCBI	16421
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration of use for clone FIB504 antibody for staining cells in flow cytometry is ≤0.5 µg per 10⁶ cells in a volume of 100 μl or 100 μl of whole blood. Each lot of this antibody is quality control tested.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	Integrin Beta 7
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors1, 2. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)3, 4. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells5, 6, subsets of dendritic cells7, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin9. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells10 and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease11.
Background	Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors^{1, 2}. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)^{3, 4}. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells^{5, 6}, subsets of dendritic cells⁷, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin⁹. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells¹⁰ and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease¹¹.
Function	Lymphocyte adhesion, hematopoietic progenitor cells migration
Other Applications Reported In Literature	B This antibody has been reported to block beta7 integrin mediated cell adhesion for In vitro and In vivo studies. IP CyTOF®
PubMed	Integrin Beta 7
Ligand/Receptor	CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Manufacturer - Research Area	Cell Biology, Immunology, Neuroscience
Manufacturer - Specificity	Clone FIB504 recognizes an epitope on human/mouse integrin β7.
RRID	AB_2830480
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Integrin α4β7 is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells, subsets of dendritic cells, and regulatory T cells (Tregs)8.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	TK1 Cells
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1141-1.0mg-safety-datasheet.pdf