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Anti-Mouse IL-12 [Clone C17.8] — Purified <i>in vivo</i> GOLD™ Functional Grade - 1.0 mg

Anti-Mouse IL-12 [Clone C17.8] — Purified <i>in vivo</i> GOLD™ Functional Grade - 1.0 mg

Item no.	LEIN-I-1175-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies Immunoprecipitation Western Blot ELISA Immunostaining
Type	Antibody Monoclonal
Applications	WB, IP, ELISA, IV
Clone	C17.8
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28 2. Ritz, J. et al. (2001) Blood. 97(12):3860-6. 3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.
NCBI	16160
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	ELISA, ICFC, in vivo, IP, N, WB
Manufacturer - Targets	IL-12
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease.
Background	IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease.
PubMed	IL-12
Manufacturer - Research Area	Immunology, Innate Immunity, Other Molecules
Manufacturer - Specificity	Clone C17.8 reacts with the p40 subunit of mouse IL-12.
RRID	AB_2737529
Concentration	2.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Purified CHO expressed Recombinant Mouse IL-12 p70
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Interleukin-12, NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF), p35, C17.8, IL-12, Mouse IL-12, Anti-Mouse IL-12 Purified Low Endotoxin Functional Formulation, CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL12, IL-12, IL-12 subunit p35, IL12A, interleukin 12 p35, interleukin 12A natural killer cell stimulatory factor 1 cytotoxic lymphocytematuration factor 1 p35, interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1,

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1175-1.0mg-safety-datasheet.pdf