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Anti-Mouse IFNAR-1 - Purified in vivo GOLD™ Functional Grade

Anti-Mouse IFNAR-1 - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-I-401-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IP, ELISA, other, IV
Clone	MAR1-5A3
Specific against	Mouse (Murine, Mus musculus)
Host	Mouse
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1.) Beaver, Jacob T. et al. (2020) Human Vaccines & Immunotherapeutics 16:9, 2092-2108 2.) Theofilopoulos, AN. et al. (2012) J Immunol. 189: 000–000. 3.) Oldstone, Michael B. A. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. 4.) Schreiber, RD et al. (2015) PLoS One.10(5):e0128636 5.) Shin, Haina et al. (2018) J Virol. 92(7): e00038-18. 6.) Crack, Peter J. et al. (2016) eNeuro 10.1523/ENEURO.0128-15.2016 7.) Sheehan, K. C. F. et al. (2006) JICR 26(11):804 8.) Dunn, G. P. et al. (2005) Nat. Immunol. 6(7):722 9.) Fenner, J. E. et al. (2006) Nat. Immunol. 7(1):33 10). Biron, C. A. et al. (2007) J Exp Med. 204(10): 2383 11.) Raju et al. (2019) Cell Reports. 29:2556–2564 12.) Ortiz et al. (2019) Cancer Cell. 35:33–45 13.) Case et al. (2020) Cell Host & Microbe. 28:465–474 14.) Hassan et al. (2020) Cell. 183:169–184 15.) Hassan et al. (2020) Cell. 182:744–753 16.) Stine et al. (2019) Cell Stem Cell. 25:830–845 17.) White et al. (2018) Cell. 175:1198–1212 18.) Jagger et al. (2017) Cell Host & Microbe. 22:366–376 19.) Richner et al. (2017) Cell. 170:273–283 20.) Richner et al. (2017) Cell. 168:1114–1125 Technical Protocols
NCBI	15975
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for clone MAR1-5A3 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. NOTE: Leinco Technologies suggests using Anti-Mouse IFNAR1-Biotin (With Streptavidin - PE; Part No. S211) (Leinco Product No.: I-402) for flow cytometry of mouse cells
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Growth Factors and Cytokines\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	IFNAR1
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	IFNAR1 is a type I membrane protein, that in conjunction with IFNAR2, makes up the heterodimeric receptor that binds all type I IFNs, which includes IFN α and β. Binding and activation of the receptor stimulates Janus protein kinases, which leads to the phosphorylation of several other proteins, namely STAT1 and STAT2. IFNAR1 has also been shown to interact with PRMT1 and Tyrosine kinase 2. Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.
Background	IFNAR1 is a type I membrane protein, that in conjunction with IFNAR2, makes up the heterodimeric receptor that binds all type I IFNs, which includes IFN α and β. Binding and activation of the receptor stimulates Janus protein kinases, which leads to the phosphorylation of several other proteins, namely STAT1 and STAT2. IFNAR1 has also been shown to interact with PRMT1 and Tyrosine kinase 2. Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.
Other Applications Reported In Literature	B Clone MAR1-5A3 has a short half-life due to the rapid recycling of cells that express the IFNAR1 receptor. In order to block function In vivo, continual blocking of all compartments is necessary. Therefore, a large loading dose is necessary to saturate all In vivo binding sites and should be maintained to ensure binding site saturation. For In vivo blocking studies, a loading dose of 2.5 mg/mouse, followed by a weekly dose of 0.5 mg/mouse is recommended. The half-life following a 2.5 mg loading dose is approximately 5 days. However, if you fail to saturate the binding sites by injecting a low dose of 250 μg, for example, the half life is only 1.5 days. WB For Western blotting, the suggested use of this reagent is 1.0 µg per ml (See Data Results) IP ELISA
PubMed	IFNAR1
Ligand/Receptor	IFN-α/β
Manufacturer - Specificity	Clone MAR1-5A3 recognizes an epitope on mouse IFNAR1.
RRID	AB_2491621
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-401-1.0mg-safety-datasheet.pdf