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Anti-Mouse CD178 (FasL) - Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD178 (FasL) - Purified in vivo PLATINUM™ Functional Grade

Item no.	LEIN-M350-5.0mg
Manufacturer	Leinco Technologies
Amount	5 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IF, other, IV
Clone	MFL3
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Purity	> 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC
Citations	1. Okumura K., et al. (1994) Proc Natl Acad Sci USA. 91:4930–4934 2. Nagata S., et al. (1995) J Immunol. 154:3806–3813 3. Saito T., et al. (1995) J Exp Med. 181:1235–1238 4. Ferguson T A., et al. (1995) Science. 270:1189–1192 5. Duke R C., et al. (1995) Nature (London). 377:630–632 6. Fink PJ. (2000) J Immunol. 165(10):5537-43 7. Matsuzawa A., et al. (1998) J Immunol. 161: 4484–4488 8. Nagata S., et al. (1998) Nat Med. 4(1):31-6 9. Hueber AO., et al (2019) Cancers (Basel). 11(5):639 10. Kabelitz D., et al. (2000) Cancer Res. 60: 822–828 11. Giannarelli D., et al. (2000) Int J Cancer. 89: 127–132 12. Kanno H., et al. (2000) Br J Cancer. 82: 1446–1452. 13. Nagano H., et al. (Cancer) Br J Cancer. 82: 1211–1217
NCBI	14103
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this CD178 antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD178
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells)1-3. Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death4. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites5, 6. FasL also contributes to CD8 proliferation and neutrophil recruitment7, 8. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling9. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer10. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis11-14.
Background	FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells)^1-3. Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death⁴. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites^{5, 6}. FasL also contributes to CD8 proliferation and neutrophil recruitment^{7, 8}. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling⁹. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer10. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis^11-14.
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Other Applications Reported In Literature	B IF
PubMed	CD178
Ligand/Receptor	Fas (CD95)
Manufacturer - Research Area	Apoptosis, Cell Biology, Cell Death, Immunology, Neuroscience, Tumor Suppressors
Manufacturer - Specificity	Clone MFL3 recognizes an epitope on mouse FasL.
RRID	AB_2894120
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	FasL is expressed on activated T cells, NK cells, the eye, and testis.
Endotoxin Level	≤ 0.5 EU/mg
Immunogen	B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-M350-5.0mg-safety-datasheet.pdf