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Anti-Mouse NKG2A/C/E - Purified in vivo GOLD™ Functional Grade

Anti-Mouse NKG2A/C/E - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-N561-50mg
Manufacturer	Leinco Technologies
Amount	50 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC, other, IV
Clone	20D5
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Vance R.E., et al. (1998) J. Exp. Med. 188:1841–1848 2. Braud VM, et al. (1998) Nature. 391(6669):795-9 3. Vance RE, et al. (1999) J Exp Med. 190(12):1801-1812 4. Brooks AG, et al. (1997) J Exp Med. 185(4):795-800 5. Gunturi A, Berg RE, Forman J. (2004) Immunol Res. 30(1):29-34 6. Bertone S, et al. (1999) Eur J Immunol. 29(1):23-9 7. Le Dréan E, et al. (1998) Eur J Immunol. 28(1):264-76 8. Lanier LL, et al. (1998) Immunity. 8(6):693-701 9. Kaiser BK, et al. (2005) J Immunol. 174(5):2878-84 10. André P, Denis C, Soulas C, et al. (2018) Cell. 175(7):1731-1743.e13 11. van Montfoort N, Borst L, Korrer MJ, et al. (2018) Cell. 175(7):1744-1755.e15
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco Flow Cytometry:For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Monoclonal Antibodies
Manufacturer - Targets	NKG2a
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Molecular Weight	150 kDa
Product Description	NKG2A/C/E antibody, clone 20D5, recognizes the NKG2A, NKG2C, and NKG2E isoforms (also known as CD159a, CD159c, m CD159e, respectively) of the NKG2 receptor, belonging to the C-type lectin-like family. NKG2 receptors are expressed at the cell surface as a heterodimer with CD94 and recognize the non-classical class I MHC-1 molecules HLA-E in humans and Qa-1 in mice1-4. NKG2A/C/E are expressed on natural killer (NK) cells, NKT cells, and activated CD8 T cells5-6. Engagement of NKG2A/CD94 transduces an inhibitory signal, blocking NK and CD8 T cell cytotoxicity and promoting self-tolerance7. In contrast, NKG2C/CD94 and NKGE/CD94 are activating receptors8 and bind with lower affinity to HLA-E9. Cancer cells frequently overexpress HLA-E to protect against NK/CD8 T cell killing, and blocking NKG2 receptors in mice promotes anti-tumor immunity and may enhance the cytotoxic potential of other therapeutic antibodies10, 11. The NKG2 receptor antibody monalizumab is currently in phase III clinical trials (INTERLINK-1) in combination with cetuximab in patients with recurrent or metastatic squamous cell carcinoma of the head and neck.
Background	NKG2A/C/E antibody, clone 20D5, recognizes the NKG2A, NKG2C, and NKG2E isoforms (also known as CD159a, CD159c, m CD159e, respectively) of the NKG2 receptor, belonging to the C-type lectin-like family. NKG2 receptors are expressed at the cell surface as a heterodimer with CD94 and recognize the non-classical class I MHC-1 molecules HLA-E in humans and Qa-1 in mice^1-4. NKG2A/C/E are expressed on natural killer (NK) cells, NKT cells, and activated CD8 T cells^5-6. Engagement of NKG2A/CD94 transduces an inhibitory signal, blocking NK and CD8 T cell cytotoxicity and promoting self-tolerance⁷. In contrast, NKG2C/CD94 and NKGE/CD94 are activating receptors⁸ and bind with lower affinity to HLA-E⁹. Cancer cells frequently overexpress HLA-E to protect against NK/CD8 T cell killing, and blocking NKG2 receptors in mice promotes anti-tumor immunity and may enhance the cytotoxic potential of other therapeutic antibodies^{10, 11}. The NKG2 receptor antibody monalizumab is currently in phase III clinical trials (INTERLINK-1) in combination with cetuximab in patients with recurrent or metastatic squamous cell carcinoma of the head and neck.
Function	Inhibitory activity via ITIMs
Other Applications Reported In Literature	IHC B
PubMed	NKG2a
Manufacturer - Research Area	Cell Biology, Immunology, Signal Transduction, Stem Cel
Manufacturer - Specificity	Clone 20D5 recognizes an epitope on the mouse NKG2A, NKG2C, and NKG2E isoforms of the CD94/NKG2 heterodimer. DBA/2J mice are CD94-deficient and do not express CD94/NKG2 receptors.
RRID	AB_2894148
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	NKG2A/C/E is expressed on NK cells, NKT cells, and activated CD8 T cells.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-N561-50mg-safety-datasheet.pdf