Item no. |
LEIN-T662-1.0mg |
Manufacturer |
Leinco Technologies
|
Amount |
1 mg |
Quantity options |
1 mg
100 mg
25 mg
5 mg
50 mg
|
Category |
|
Type |
Antibody Monoclonal |
Applications |
FC, IP, IHC, IV |
Clone |
H57-597 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Hamster - Armenian |
Purity |
> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC |
Citations |
1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7 |
NCBI |
21577 |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Quality Tested by Leinco FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. |
Manufacturer - Category |
Purified in vivo Functional Grade>Gold Purified Bulk Antibodies|PhenoCycler®|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers |
Manufacturer - Targets |
TCR beta |
Country of Origin |
USA |
Shipping Temperature |
Next Day 2-8°C |
Storage Conditions |
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Product Description |
TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3, 4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5, 6. |
Background |
TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3, 4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5, 6. |
Function |
Antigen recognition, T cell activation |
Additional Reported Applications For Relevant Conjugates |
CODEX® |
Other Applications Reported In Literature |
Costim Depletion IP IHC FF IHC FFPE |
PubMed |
TCR beta |
Ligand/Receptor |
Peptide bound-MHC class I and II |
Manufacturer - Specificity |
Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells. |
RRID |
AB_2832104 |
Concentration |
≥ 5.0 mg/ml |
Formulation |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
Product Preparation |
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Antigen Distribution |
TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells. |
Endotoxin Level |
≤ 1.0 EU/mg |
Immunogen |
Affinity purified TCR from mouse DO-11.10 cells |
Additional Information |
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.