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Anti-Mouse TCR β chain - Purified in vivo GOLD™ Functional Grade

Anti-Mouse TCR β chain - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-T662-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IP, IHC, IV
Clone	H57-597
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7
NCBI	21577
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|PhenoCycler®\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	TCR beta
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3, 4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5, 6.
Background	TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells¹. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond². TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling^{3, 4}. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival^{5, 6}.
Function	Antigen recognition, T cell activation
Additional Reported Applications For Relevant Conjugates	CODEX®
Other Applications Reported In Literature	Costim Depletion IP IHC FF IHC FFPE
PubMed	TCR beta
Ligand/Receptor	Peptide bound-MHC class I and II
Manufacturer - Specificity	Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
RRID	AB_2832104
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Affinity purified TCR from mouse DO-11.10 cells
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T662-1.0mg-safety-datasheet.pdf