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Anti-Mouse CD120b (TNFR2) (Clone TR75-54.7) - Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD120b (TNFR2) (Clone TR75-54.7) - Purified in vivo PLATINUM™ Functional Grade

Item no.	LEIN-T954-5.0mg
Manufacturer	Leinco Technologies
Amount	5 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IP, ELISA, other, IV
Clone	TR75-54.7
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Purity	> 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC
Citations	1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217. 2. Sheehan, KC. et al. (1995) J. Exp. Med. 181:607.
NCBI	21938
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for clone TR75-54.7 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines\|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Purified in vivo Functional Grade
Manufacturer - Targets	TNFR2
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	Tumor necrosis factor receptor II (TNF-RII) is present on most cell types1 and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.2
Background	Tumor necrosis factor receptor II (TNF-RII) is present on most cell types¹ and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.²
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Function	Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Other Applications Reported In Literature	IP B WB ELISA Cap
PubMed	TNFR2
Ligand/Receptor	TNF-α, LT-α (TNF-β)
Manufacturer - Research Area	Immunology, Innate Immunity
Manufacturer - Specificity	Anti-Mouse CD120 recognizes Mouse CD120. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2832125
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Variety of cell types at low levels
Endotoxin Level	≤ 0.5 EU/mg
Immunogen	Purified Recombinant Mouse TNF R2
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T954-5.0mg-safety-datasheet.pdf