ELISA
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Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) | Login | Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) | ||||||||||||||||
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Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) | Login | Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) | ||||||||||||||||
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Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) | Login | Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) | ||||||||||||||||
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Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) | Login | Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) | ||||||||||||||||
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Human Soluble NT5E/CD73 ELISA Kit, PRO | Login | Human Soluble NT5E/CD73 ELISA Kit, PRO | ||||||||||||||||
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Human Soluble NT5E/CD73 ELISA Kit, PRO | Login | Human Soluble NT5E/CD73 ELISA Kit, PRO | ||||||||||||||||
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resDetect™ Human Interleukin-21 (IL-21) ELISA Kit (Residue Testing) | Login | resDetect™ Human Interleukin-21 (IL-21) ELISA Kit (Residue Testing) | ||||||||||||||||
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resDetect™ Human Interleukin-2 (IL-2) ELISA Kit (Residue Testing) | Login | resDetect™ Human Interleukin-2 (IL-2) ELISA Kit (Residue Testing) | ||||||||||||||||
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resDetect™ Human Interleukin-6 (IL-6) ELISA Kit (Residue Testing) | Login | resDetect™ Human Interleukin-6 (IL-6) ELISA Kit (Residue Testing) | ||||||||||||||||
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resDetect™ Human Interleukin-15 (IL-15) ELISA Kit (Residue Testing) | Login | resDetect™ Human Interleukin-15 (IL-15) ELISA Kit (Residue Testing) | ||||||||||||||||
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resDetect™ Human Interleukin-4 (IL-4) ELISA Kit (Residue Testing) | Login | resDetect™ Human Interleukin-4 (IL-4) ELISA Kit (Residue Testing) | ||||||||||||||||
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200x DDM CHS buffer | Login | 200x DDM CHS buffer | ||||||||||||||||
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resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) | Login | resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) | ||||||||||||||||
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HEK293/Human TrkB Stable Cell Line | Login | HEK293/Human TrkB Stable Cell Line | ||||||||||||||||
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NY-ESO-1 specific TCR-HEK293 cell line | Login | NY-ESO-1 specific TCR-HEK293 cell line | ||||||||||||||||
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HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line | Login | HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line | ||||||||||||||||
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HEK293/Human Anti-CD19 Stable Cell Line | Login | HEK293/Human Anti-CD19 Stable Cell Line | ||||||||||||||||
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HEK293/Human ASGR1 Stable Cell Line | Login | HEK293/Human ASGR1 Stable Cell Line | ||||||||||||||||
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HEK293/Human GPRC5D Stable Cell Line | Login | HEK293/Human GPRC5D Stable Cell Line | ||||||||||||||||
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HEK293/Human Claudin-18.2 Stable Cell Line | Login | HEK293/Human Claudin-18.2 Stable Cell Line | ||||||||||||||||
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HEK293/Human CD20 Stable Cell Line | Login | HEK293/Human CD20 Stable Cell Line | ||||||||||||||||
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HEK293/Human CCR5 Stable Cell Line | Login | HEK293/Human CCR5 Stable Cell Line | ||||||||||||||||
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HEK293/Human FcRn (FCGRT & B2M) Stable Cell Line | Login | HEK293/Human FcRn (FCGRT & B2M) Stable Cell Line | ||||||||||||||||
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HEK293/Human 4-1BB / TNFRSF9 Stable Cell Line | Login | HEK293/Human 4-1BB / TNFRSF9 Stable Cell Line | ||||||||||||||||
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HEK293/Human 4-1BB Ligand / TNFSF9 Stable Cell Line | Login | HEK293/Human 4-1BB Ligand / TNFSF9 Stable Cell Line | ||||||||||||||||
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Enzyme Linked Immunosorbent Assay.
For the detection of proteins, viruses or low molecular weight compounds (for example: hormones, pesticides and toxins) and their concentration determination the Enzyme Linked Immunosorbent Assay is used.
n this enzyme immunoassays (EIA) a polystyrene multiwell plate is coated with a specific antibody (capture antibody) against the sample (analyte or antigens). This immobilized antibody binds a specific amino acid sequence (epitope) on the analyte. This is followed by incubation with a second antibody (detection antibody) which can be both monoclonal origin (from hybridoma cells) as well as polyclonal (from animal serum). If these antibodies are already conjugated to a fluorophore or an enzyme, the detection can be done directly. In the event that the second antibody is uncoupled a further conjugated antibody is necessary for the detection (indirect ELISA). Thus, a characteristic color change after the addition of a substrate or a monochromatic excitation (in the case of a fluorophore-conjugated antibody) can be achieved.
The assay formats can also be divided into direct and indirect ELISA test, sandwich ELISA test and competitive or inhibitory ELISA test.
An ELISA kit offers you additional flexibility, by the ability to coat the polystyrene multiwell plate on your own.
Descriptive information about ELISA is available in our Data Library.