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ELISA
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| Dog C-Reactive Protein (CRP) ELISA kit | Login | Dog C-Reactive Protein (CRP) ELISA kit | ||||||||||||||||
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| Rat Anti-Dinitrophenol (DNP) IgG ELISA kit | Login | Rat Anti-Dinitrophenol (DNP) IgG ELISA kit | ||||||||||||||||
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| Rat Anti-Dinitrophenol (DNP) IgM ELISA kit | Login | Rat Anti-Dinitrophenol (DNP) IgM ELISA kit | ||||||||||||||||
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| Rat Fibrinogen ELISA kit | Login | Rat Fibrinogen ELISA kit | ||||||||||||||||
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| Rat Gc-globulin ELISA kit | Login | Rat Gc-globulin ELISA kit | ||||||||||||||||
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| Camel Haptoglobin ELISA kit | Login | Camel Haptoglobin ELISA kit | ||||||||||||||||
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| Rat Haptoglobin ELISA kit | Login | Rat Haptoglobin ELISA kit | ||||||||||||||||
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| Dog Haptoglobin ELISA kit | Login | Dog Haptoglobin ELISA kit | ||||||||||||||||
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| Pig Haptoglobin ELISA kit | Login | Pig Haptoglobin ELISA kit | ||||||||||||||||
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| Rabbit Cardiac Fatty Acid Binding Protein (H-FABP) ELISA kit | Login | Rabbit Cardiac Fatty Acid Binding Protein (H-FABP) ELISA kit | ||||||||||||||||
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| Dog Cardiac Fatty Acid Binding Protein (H-FABP) ELISA kit | Login | Dog Cardiac Fatty Acid Binding Protein (H-FABP) ELISA kit | ||||||||||||||||
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| Sheep IgA ELISA kit | Login | Sheep IgA ELISA kit | ||||||||||||||||
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| Goat IgA ELISA kit | Login | Goat IgA ELISA kit | ||||||||||||||||
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| Horse IgA ELISA kit | Login | Horse IgA ELISA kit | ||||||||||||||||
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| Rat IgA ELISA kit | Login | Rat IgA ELISA kit | ||||||||||||||||
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| Pig IgA ELISA kit | Login | Pig IgA ELISA kit | ||||||||||||||||
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| Mouse IgG1 ELISA kit | Login | Mouse IgG1 ELISA kit | ||||||||||||||||
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| Mouse IgG2a ELISA kit | Login | Mouse IgG2a ELISA kit | ||||||||||||||||
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| Mouse IgG3 ELISA kit | Login | Mouse IgG3 ELISA kit | ||||||||||||||||
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| Rabbit IgG ELISA kit | Login | Rabbit IgG ELISA kit | ||||||||||||||||
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| Goat IgG ELISA kit | Login | Goat IgG ELISA kit | ||||||||||||||||
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| Horse IgG(T) ELISA kit | Login | Horse IgG(T) ELISA kit | ||||||||||||||||
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| Rat IgG ELISA kit | Login | Rat IgG ELISA kit | ||||||||||||||||
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| Rat IgG2a ELISA kit | Login | Rat IgG2a ELISA kit | ||||||||||||||||
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| Monkey IgG ELISA kit | Login | Monkey IgG ELISA kit | ||||||||||||||||
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Enzyme Linked Immunosorbent Assay.
For the detection of proteins, viruses or low molecular weight compounds (for example: hormones, pesticides and toxins) and their concentration determination the Enzyme Linked Immunosorbent Assay is used.
n this enzyme immunoassays (EIA) a polystyrene multiwell plate is coated with a specific antibody (capture antibody) against the sample (analyte or antigens). This immobilized antibody binds a specific amino acid sequence (epitope) on the analyte. This is followed by incubation with a second antibody (detection antibody) which can be both monoclonal origin (from hybridoma cells) as well as polyclonal (from animal serum). If these antibodies are already conjugated to a fluorophore or an enzyme, the detection can be done directly. In the event that the second antibody is uncoupled a further conjugated antibody is necessary for the detection (indirect ELISA). Thus, a characteristic color change after the addition of a substrate or a monochromatic excitation (in the case of a fluorophore-conjugated antibody) can be achieved.
The assay formats can also be divided into direct and indirect ELISA test, sandwich ELISA test and competitive or inhibitory ELISA test.
An ELISA kit offers you additional flexibility, by the ability to coat the polystyrene multiwell plate on your own.
Descriptive information about ELISA is available in our Data Library.
