mAbMods™ Anti-Mouse CD20 (Clone 18B12) - 5.0 mg

Recombinant Antibodies>Chimeric Antibody|Purified in vivo Functional Grade|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers

2 - 8°C Wet Ice

This anti-CD20 antibody is a recombinant chimeric version of the original clone 18B12 antibody. The variable domain sequences are identical to the original clone 18B12; however, the constant region sequences have been switched from mouse IgG1 to mouse IgG2a.CD20 is a phosphoprotein belonging to the MS4A family, with a size of 33-37 kD. It is primarily found on B cells, including malignant B cells. This protein serves as a target, for therapies that aim to deplete B cells using antibodies 1–4. CD20 works alongside surface IgM and plays a role in calcium conductance, cell cycle progression, and B cell growth 5, 6. Many B cell malignancies exhibit levels of CD20, which remain on the cell surface after treatment with monoclonal antibodies. This makes it an effective target for antibody-based treatments 7.Although CD20 is mainly associated with B cells it has also been observed on a subset of T lymphocytes and follicular dendritic cells. However, its function in these cell types is not yet fully understood. CD20-positive T cells have been detected in conditions like autoimmune diseases, cancers, and infections 8, 9.Clone 18B12 is commonly used in research for studying B cell biology and immune responses. Researchers utilize this antibody for applications like flow cytometry analysis, immunohistochemistry studies as well as in vivo investigations using mouse models. By employing this antibody variant scientists have made progress in understanding the role of CD20, in autoimmune diseases, lymphomas, and other disorders related to B cell dysfunction 10–14.

≥ 5.0 mg/ml

CD20 is a general B cell marker expressed by most normal B cells in all stages of their development and by most B cell malignancies.