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QuantiChrom LDH Cytotoxicity Assay Kit (C2LD-100)

QuantiChrom LDH Cytotoxicity Assay Kit (C2LD-100)

Item no.	BAS-C2LD-100
Manufacturer	BioAssay Systems
Amount	100 Tests
Quantity options	100 Tests 100x100 Tests 20x100 Tests
Category	Cell Biology Cell Status Toxicity & Cytotoxicity Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Citations	Vergis, J et al (2021). Exploring Galleria mellonella larval model to evaluate antibacterial efficacy of Cecropin A (1-7)- Melittin against multi-drug resistant enteroaggregative Escherichia coli. Pathogens and Disease, ftab010. Assay: LDH Cytotoxicity in galleria mellonella. Zhou, et al (2019). Retrospective analysis of the efficacy of adjuvant CIK cell therapy in epithelial ovarian cancer patients who received postoperative chemotherapy. OncoImmunology, 8(2), e1528411. Assay: LDH Cytotoxicity in human cells. Flage, B. (2018). Heme-induced acute lung injury by inflammasome activation in vascular endothelium (Doctoral dissertation, University of Pittsburgh). Assay: Cytotoxicity in human tissues. To find more recent publications, please click here.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Description	Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 2 U/L. Shelf life: 6 months. Shipping: ambient tem
Faqs	Does this assay kit work for a particular cell line? Yes, this kit works for all cell lines of any species. Why is there no standard for this assay? This assay calculates the percentage of cell death. Therefore it uses the Total Lysis well and the No Treatment Control well (instead of a standard) to calculate the percentage of cell death in your samples. How long do I need to incubate my test compounds with my cells? This depends entirely on your test compound and your experiment. The incubation can be as short as 5 minutes to as long as one day. The Triton-X 100 total lysis reagent acts immediately to lyse the cells and can be read immediately if so desired. What is the best way to store the reagent? If you will be using the assay constantly every day, then store in the refridgerator. For long term storage, freeze the reagent at -20C. When does the assay expire? The shelf life of the assay is at least one year after receipt at -20C. For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview	Application For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis. Key Features Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure take 20 min. Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day. Method OD500nm Samples Cell culture Species All Size 100 tests Shelf Life 12 months (-20C) More Details Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.
Shipment	RT
Storage	4C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 Tests

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Delivery expected until 9/25/2025

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