Can you use this assay with serum or plasma samples?
Yes, serum and plasma samples should be diluted 10-fold in dH
2O prior assay. Correct for dilution factor (n = 10) in the calculation.
Can you use this assay with urine samples?
Yes, urine samples should be diluted 20-fold in dH
2O prior assay. Correct for dilution factor (n = 20) in the calculation.
What happens if my sample has oxidants or reactive oxygen species (ROS) in it?
Samples with oxidants or ROS will yield lower measurements of antioxidant potential because antioxidants in the sample will reduce the oxidants and ROS rather than the Fe
3+, this results in lower levels of Fe
2+, which is what the assay measures to determine FRAP.
What happens if my sample has iron in it?
This assay measures antioxidant potential to reduce Fe
3+ to Fe
2+. If the sample has intrinsic Fe
2+, this will yield a false positive for antioxidant potential.
For more detailed product information and questions, please feel free to
Contact Us. Or for more general information regarding our assays, please refer to our
General Questions.