EnzyChrom Adipolysis Assay Kit

Application

• For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.

Key Features

• Sensitive and accurate. Use as little as 10 ?L samples. Linear detection range in 96-well plate: 0.92 to 100 ?g/mL (10 to 1000 ?M) glycerol for colorimetric assays and 0.2 to 5 ?g/mL for fluorimetric assays.
• Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
• Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.

Method

• OD570nm, or FL530/585nm

Samples

• Cell culture media

Species

• All

Size

• 200 tests

Detection Limit

• 0.92 ?g/mL

Shelf Life

• 12 months

More Details

• Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. BioAssay Systems adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.