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EnzyChrom Adipolysis Assay Kit

EnzyChrom Adipolysis Assay Kit

Item no.	BAS-EAPL-200
Manufacturer	BioAssay Systems
Amount	200 Tests
Quantity options	200 Tests 100x200 Tests 20x200 Tests
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Citations	Nascimento, EBM et al (2021). Nicotinamide riboside enhances in vitro beta-adrenergic brown adipose tissue activity in humans. The Journal of Clinical Endocrinology and Metabolism. Assay: Adipolysis in human adipocytes. Huttala, Outi, et al (2019). Presence of vasculature results in faster insulin response in adipocytes in vascularized adipose tissue model. ALTEX-Alternatives to animal experimentation. Assay: Adipolysis in human adipose tissue. Jocken, Johan WE, et al (2018). Short-chain fatty acids differentially affect intracellular lipolysis in a human white adipocyte model. Frontiers in endocrinology 8: 372. Assay: Adipolysis in human stem cell. Wang, Rebecca Y., et al (2017). Development of a three-dimensional adipose tissue model for studying embryonic exposures to obesogenic chemicals. Annals of biomedical engineering 45.7: 1807-1818. Assay: Adipolysis in human tissues. Zheng, Qiantao, et al (2017). Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity. Proceedings of the National Academy of Sciences 114.45: E9474-E9482. Assay: Adipolysis in pig tissues. Huttala, Outi, et al (2016). Differentiation of human adipose stromal cells in vitro into insulin-sensitive adipocytes. Cell and tissue research 366.1: 63-74. Assay: Adipolysis in human cells. Kim, Hyo Joon, et al (2016). An apolipoprotein B100 mimotope prevents obesity in mice. Clinical Science 130.2: 105-116. Assay: Adipolysis in mouse cells. Yuan, Xiaoxue, et al (2016). Rutin ameliorates obesity through brown fat activation. The FASEB Journal 31.1: 333-345. Assay: Adipolysis in mouse cells. Chandrasekaran V., Amit A. et al (2013). Effect of Nelumbo nucifera Petal Extracts on Lipase, Adipogenesis, Adipolysis, and Central Receptors of Obesity. Evidence-Based Complementary and Alternative Medicine. 2013: 10.1155/2013/145925 Assay: Adipolysis in cell lines. Li R et al (2012). Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes. Regul Pept. 178(1-3):16-20. Assay: Adipolysis in mouse adipocytes. To find more recent publications, please click here.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Description	Quantitative assay of adipolysis by measuring glycerol released in cell culture using colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 20 min. Kit size: 200 tests. Detection limit: 0.92 ug/mL. Shelf life: 6 months. Shipping: on ice;
Faqs	I collected the medium from the culture wells. Do I need to now centifuge the medium and collect the supernatant or do I use the medium itself for the assay? You can assay the cell culture medium directly. However, if it contains cellular debris you should first centrifuge it and then use the clear supernatant in the assay. What is the principle of the assay? In the process of adipolysis triglycerides are hydrolyzed by the cellular lipases into glycerol and free fatty acids. The assay measures glycerol, the end product of adipolysis, in a coupled three step enzyme reaction: 1) Glycerol kinase converts glycerol and ATP to glycerol phosphate and ADP. 2) Glycerol phosphate oxidase converts glycerol phosphate and O₂ to glycerone phosphate and H₂O₂ 3) HRP converts ADHP and H₂O₂ to resorufin and water. For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview	Application For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features Sensitive and accurate. Use as little as 10 ?L samples. Linear detection range in 96-well plate: 0.92 to 100 ?g/mL (10 to 1000 ?M) glycerol for colorimetric assays and 0.2 to 5 ?g/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method OD570nm, or FL530/585nm Samples Cell culture media Species All Size 200 tests Detection Limit 0.92 ?g/mL Shelf Life 12 months More Details Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. BioAssay Systems adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.
Shipment	ICE
Storage	-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 Tests

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Delivery expected until 9/25/2025

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