EnzyChrom Monoamine Oxidase Assay Kit

Application

• For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified.
• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585nm

Samples

• MAO enzyme preparations

Species

• All

Size

• 100 tests

Detection Limit

• 0.01 U/L

Shelf Life

• 6 months

More Details

• Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression. BioAssay Systems' MAO Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H₂O₂, which is determined by a fluorimetric method (?ex/em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.