EnzyChrom Nitric Oxide Synthase Assay Kit

Application

• For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.

Key Features

• Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate.
• Rapid and reliable. Can be completed in 40 min if reduction of NO₃^- to NO₂^- is performed at 60C.

Method

• OD540nm

Samples

• Biological

Species

• All

Size

• 100 tests

Detection Limit

• 0.25 U/L

Shelf Life

• 6 months

More Details

• Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in Research and Drug Discovery. BioAssay Systems EnzyChrom Nitric Oxide Synthase Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 min.