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EnzyChrom Phospholipase D Assay Kit

EnzyChrom Phospholipase D Assay Kit

Manufacturer	BioAssay Systems
Category	Cardiovascular Research Thrombosis & Hemostasis Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Amount	100 Tests
Item no.	BAS-EPPD-100
eClass 6.1	32161000
eClass 9.0	32161090
Available
Description	Quantitative determination of phospholipase D activity by colorimetric (570nm) or fluorimetric (530/590nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.04 U/L. Shelf life: 3 months. Shipping: on ice; storage: -20C.
Faqs	I was curious how much PLD protein was used per well to generate the two data graphs shown in the product information for the kit. The specific activity of an enzyme in terms of U/mg varies with the preparation and can also decrease over time. Our current phospholipase D (from Streptomyces sp.) has a specific activity of 6 U/mg. So if we want to reproduce the graph on the datasheet, we need to use 1.67 mg of our phospolipase D to obtain the 10/UL data point. The specific activity of your enzyme preparation may be much lower (or higher) and you should to titrate the preparation starting at the highest possible concentration. For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview	Application For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism. Key Features Sensitive. Use 10 ?L samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Method OD570nm, or FL530/585nm Samples Biological Species All Size 100 tests Detection Limit 0.04 U/L Shelf Life 6 months More Details Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. BioAssay Systems method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H₂O₂ specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
Shipment	ICE
Storage	-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BAS-EPPD-100-datasheet.pdf

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Amount: 100 Tests

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Delivery expected until 5/30/2024