EnzyChrom™ Sialic Acid Assay Kit

ICE

For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µ L samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µ M for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µ M.

Application: For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
Key Features: Sensitive and accurate. Use as little as 10 ?L samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 ?M for fluorimetric assays.
Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step.
Method: OD570nm, or FL530/585nm
Samples: Biological
Species: All
Size: 100 tests
Detection Limit: OD, FL: 20, 2 ?M
Shelf Life: 6 months
More Details: Sialic acid is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The Sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple, direct and automation-ready procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. BioAssay Systems sialic acid assay uses a single Working Reagent that combines NANA aldolase, pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at ?ex/em = 530/585nm is directly proportional to sialic acid concentration in the sample.