« Back
Home /
QuantiFluo Diamine Oxidase Assay Kit

QuantiFluo Diamine Oxidase Assay Kit

Item no.	BAS-QFDO-100
Manufacturer	BioAssay Systems
Amount	100 Tests
Quantity options	100 Tests 100x100 Tests 20x100 Tests
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Description	Quantitative determination of diamine oxidase activity by fluorimetric (530nm/585nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L. Shelf life: 6 months. Shipping: ambient temp; storage: -20C.
Faqs	How do I store the kit? This kit is shipped at room temperature. Upon receiving, please keep the kit in the freezer (-20 to -10C) for long term stability. What samples have you tested? Serum samples were tested. Since DAO is copper dependent, EDTA should be avoided in sample preparation. What is the benefit of using an internal standard? Serum and plasma samples greatly influence the HRP reaction. In order to measure an appropriate ?RFU for H₂O₂ in a serum or plasma matrix, an internal standard is required. Will the assay kit work in a 384 well plate, cuvette, etc? Yes, the assay can be used in any standard black plate or cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 ?L, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 ?L). Can I store unused reagents for future use? Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 1 hour. I don’t have the correct wavelength filter, will the assay still work? Typically if you are within +/- 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify. For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview	Application For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc) Key Features Fast and sensitive. Use of 10 ?L sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25C. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method FL530/585nm Samples Serum, Plasma Species All Size 100 tests Detection Limit 0.5 U/L Shelf Life 6 months More Details DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT. BioAssay Systems non-radioactive, fluorimetric DAO assay is based on the oxidation of putrescine to pyrroline plus NH₃ and H₂O₂. The generated H₂O₂ is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at ?ex/em = 530/585 nm is directly proportional to the enzyme activity. - Enzyme-Activity-Assays.htm" target="_blank"> Other Enzyme Activity Assay Kits - Metabolism.htm" target="_blank"> Other Metabolism Assay Kits
Shipment	RT
Storage	-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BAS-QFDO-100-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 100 Tests

available

Delivery expected until 9/25/2025

Express Delivery: Save 1 days

Compare

Add to wishlist

Get an offer