Anti-Neuron-Specific Enolase (NSE) Type 2 Antibody

Primary Antibody

Neuron-Specific Enolase (NSE) Type 2

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

200 µg/mL

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Regular vials contain 1000 µ, l of this antibody mixture, Sampler vials contain 200 ul of this mixture.

We produce our Anti-Neuron-Specific Enolase (NSE) Type 2 chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

AB_2313555

1:10000-120000

1:10000-1:20000

Human, Mouse, Rat

12 months from receipt of product

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.