Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1)

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Wet Ice

The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that it will work on other mammalian tissues.
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein.
Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (cat. #OAPC00025)(green). The large cells are alpha -motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.

Liquid. Contains 10 mM sodium azide.

Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.

NP_004172

Monoclonal

Recombinant full length human UCHL1 purified from E. coli.

Specific for the ~24kDa UCHL1 protein.